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KMID : 0881720100250020118
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Journal of Food Hygiene and Safety
2010 Volume.25 No. 2 p.118 ~ p.121
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Determination of six Sweeteners in children`s Favorite Foods by HPLC-MS/MS
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Kim Il-Young
Du Ok-Ju
Lee Sung-Deuk
Park Young-Hye
Kim Mi-Sun
Bae Chung-Ho
Chae Young-Zoo
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Abstract
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A HPLC-MS/MS method was developed for simultaneous determination of six sweeteners (acesulfame- K, cyclamate, saccharin, sucralose, stevioside, aspartame) in children’s favorite foods. The procedure involves an extraction of the six sweeteners with 50% methanol solution, sample clean-up using the Carrez clearing reagent and filtering with cartridge filter. The HPLC separation was performed on a Hypersil Gold (150 mm × 2.1 mm 5 um) column using the water/acetonitrile mobile phase (95:5). Mass spectrometric analysis was carried out using the TSQ Quantum Ultra operated in negative and positive ESI/SRM. With this method, good linear relationship, sensitivity and reproducibility were obtained. The spike recoveries of six sweeteners for 2 kinds of foods spiked into 0.4 mg/ kg ranged from 87.4 to 114.7%. The detection limits were above 0.02 mg/kg. The method has been applied to determination of six sweeteners in children’s favorite foods.
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KEYWORD
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Sweeteners, ESI, children`s favorite foods
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